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Clean 'MSnExp', 'Spectrum' or 'Chromatogram' instances

clean-methods.Rd

This method cleans out individual spectra (Spectrum instances), chromatograms (Chromatogram instances) or whole experiments (MSnExp instances) of 0-intensity peaks. Unless all is set to FALSE, original 0-intensity values are retained only around peaks. If more than two 0's were separating two peaks, only the first and last ones, those directly adjacent to the peak ranges are kept. If two peaks are separated by only one 0-intensity value, it is retained. An illustrative example is shown below.

Methods

signature(object = "MSnExp", all = "logical", verbose = "logical")

Cleans all spectra in MSnExp object. Displays a control bar if verbose set to TRUE (default). Returns a cleaned MSnExp instance.

signature(object = "Spectrum", all = "logical", msLevel. = "numeric")

Cleans the Spectrum object. Returns a cleaned Spectrum instance. If all = TRUE, then all zeros are removed. msLevel. defines the level of the spectrum, and if msLevel(object) != msLevel., cleaning is ignored. Only relevant when called from OnDiskMSnExp and is only relevant for developers.

signature(object = "Chromatogram", all = "logical", na.rm = "logical")

Cleans the Chromatogram instance and returns a cleaned Chromatogram object. If na.rm is TRUE (default is FALSE) all NA intensities are removed before cleaning the chromatogram.

Author

Laurent Gatto

See also

removePeaks and trimMz for other spectra processing methods.

Examples

int <- c(1,0,0,0,0,0,0,0,1,1,1,0,0,0,0,0,1,1,0,0,0,0,0,0,0,0,0,0,1,0,0,0)
sp1 <- new("Spectrum2",
           intensity=int,
           mz=1:length(int))
sp2 <- clean(sp1) ## default is all=FALSE
intensity(sp1)
#>  [1] 1 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0
intensity(sp2)
#>  [1] 1 0 0 1 1 1 0 0 1 1 0 0 1 0
intensity(clean(sp1, all = TRUE))
#> [1] 1 1 1 1 1 1 1

mz(sp1)
#>  [1]  1  2  3  4  5  6  7  8  9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25
#> [26] 26 27 28 29 30 31 32
mz(sp2)
#>  [1]  1  2  8  9 10 11 12 16 17 18 19 28 29 30
mz(clean(sp1, all = TRUE))
#> [1]  1  9 10 11 17 18 29

data(itraqdata)
itraqdata2 <- clean(itraqdata)
sum(peaksCount(itraqdata))
#> [1] 106289
sum(peaksCount(itraqdata2))
#> [1] 59747
processingData(itraqdata2)
#> - - - Processing information - - -
#> Data loaded: Wed May 11 18:54:39 2011 
#> Updated from version 0.3.0 to 0.3.1 [Fri Jul  8 20:23:25 2016] 
#> Spectra cleaned: Tue Aug 26 11:38:13 2025 
#>  MSnbase version: 1.1.22 

## Create a simple Chromatogram object
chr <- Chromatogram(rtime = 1:12,
                    intensity = c(0, 0, 20, 0, 0, 0, 123, 124343, 3432, 0, 0, 0))

## Remove 0-intensity values keeping those adjacent to peaks
chr <- clean(chr)
intensity(chr)
#> [1]      0     20      0      0    123 124343   3432      0

## Remove all 0-intensity values
chr <- clean(chr, all = TRUE)
intensity(chr)
#> [1]     20    123 124343   3432

## Clean a Chromatogram with NAs.
chr <- Chromatogram(rtime = 1:12,
                    intensity = c(0, 0, 20, NA, NA, 0, 123, 124343, 3432, 0, 0, 0))
chr <- clean(chr, all = FALSE, na.rm = TRUE)
intensity(chr)
#> [1]      0     20      0    123 124343   3432      0